TEACHING AND LABORATORY EXPERIENCE

• Teaching-learning activities of undergraduate (MBBS, BDS, B.Sc. Nursing, B.N, BMIT, B.Sc. MLT, BPH and B. Pharmacy) students;
• Teaching Clinical Microbiology, Immunology, infectious diseases and applied aspects to postgraduate MSc Microbiology and MD/MS residents;
• Supervised thesis for postgraduate and undergraduate students.
• Conducting Problem Based Learning (PBL) sessions for undergraduate MBBS 1st and 2nd year students;
• Diagnostics: reporting and microbiological analysis of various clinical specimens in the Clinical Laboratory;
• Perform and Check day-to-day routine investigations in hospital. Monitor quality control both internal and external of hospital Microbiology laboratory and 
• Researches

TECHNICAL EXPERTISE

• Manual media preparation of basic and complex media, sterilization and checking efficiency of sterilization.
• Bacteriology - Clinical Specimen Collection, Processing, Antibiotic Sensitivity Testing, Reporting
• Anaerobic Bacteriology - Clinical specimen collection, Processing, Operation of Anaerobic jar, Reporting
• Mycobacteriology - Specimen Collection, Processing and Culture
• Virology – tissue culture
• Mycology- Collection of Clinical Specimen, Processing, Culture and Reporting
• Parasitology - Collection of Clinical Specimen, Processing, Culture and Reporting
• Serology
  • Western Blot for HIV, TORCH Panel, WIDAL for Typhoid, Serotyping 
  • Brucella Agglutination Test
  • Weil-Felix for Rickettsial Diseases
  • Syphilis (VDRL, RPR, TPHA)
  • ELISA (HIV, HCV, ANA), CRP, ASO, Paul Bunnel, RF, IgM for Leptospirosis)
  • Spot- Test (HIV TriDot, HCV TriDot, HBsAg)
  • Automated Blood Culture Systems (BACTEC)
  • Sterility checking of Operation Theater, ICU, NICU.
  • Bacteriological Examination of water and evaluation of efficacy of disinfectants.

• Conventional PCR Machine.
• Real time- PCR 
• Multiplex PCR
• Vitek-2
• BACTEC
• Agarose gel electrophoresis and Gel doc system
• Fluorescence microscope
• Inspissator

1. Bhatta DR, Gokhale S, Sharma AL, Gupta U, Gaur A, Gowda S,  S Raut , Thapa S, Khadka R. Carrier state of Haemophilus influenzae type b (Hib), S. pneumoniae, S. pyogenes, N. meningitidis and C. diphtheriae among school children in Pokhara, Nepal. Asian Pacific Journal of Tropical Diseases 2014; 4(1): 45-9. doi:10.1016/S2222-1808(14)60312-1
2. Ansari S, Paudel P, Gautam K, Shrestha S, Thapa S, Gautam R. Chromobacterium violaceum Isolated from a Wound Sepsis: A Case Study from Nepal. Case Report in Infectious Disease 2015:181946. doi: 10.1155/2015/181946. 
3. Sapkota LB, Thapa S. Dipeptidyl-Peptidase IV Inhibitors and glycemic control in type 2 Diabetes Mellitus. Journal of the College of Medical Sciences 2016;12(1):28-32. DOI: http:// dx.doi.org/10.3126/ jcmsn.v12i1.14683.
4. Neopane P, Nepal HP,  Gautam R, Thapa S, Adhikary BR, S Rojeet, A Yoshihiro. Detection of Biofilm Formation by Acinetobacter baumanii Isolates and Its Association with Multidrug Resistance. LMCE & KSLM Laboratory Medicine Congress & Exhibition, The Korean Society for Laboratory Medicine. 2016; 36(1).
5. Ansari S, Dhital R, Shrestha S, Thapa S, Puri R, Chaudhary N, Khatiwada S, Gautam R. “Growing Menace of Antibacterial Resistance in Clinical Isolates of Pseudomonas aeruginosa in Nepal: An Insight of Beta-Lactamase Production,” BioMed Research International 2016; 1-8. https://doi.org/10.1155/2016/6437208.
6. Thapa S, Sapkota LB. Prevalence of inducible clindamycin resistance in erythromycin resistant clinical isolates of Staphylococcus aureus and CONS at tertiary care hospital. Journal of the College of Medical Sciences 2016;12(3):83-88. https://doi.org/10.3126/jcmsn.v12i3.16011
7. Hosuru Subramanya S, Thapa S, Dwedi SK, Gokhale S, Sathian B, Nayak N, Bairy I. Streptococcus pneumoniae and Haemophilus species colonization in health care workers: the launch of invasive infections? BMC Res Notes. 2016;9:66. doi: 10.1186/s13104-016-1877-x.
8. Neopane P , Nepal HP,  Gautam R, Paudel R, Shamshul Ansari S, Sony Shrestha S, Thapa S. Is there Correlation of Biofilm formation with Multidrug Resistance and ESBL Production in Pseudomonas aeruginosa? European Journal of Biomedical and Pharmaceutical Sciences 2017; 4(1):366-372. www.ejbps.com
9. Thapa S, Sapkota LB. Burden of Dengue Virus Infection in Chitwan and Peripheral Districts: An Emerging Disease in Terai Region of Nepal. International Journal of Tropical Disease and Health 2016;21(1):1-10. http://www.sciencedomain.org/review-history/17590.
10. Sapkota LB, Thapa S. Assessment of serum electrolytes in hypothyroid patients attending Chitwan medical college teaching hospital. Asian Journal of Medicine and Health 2017;2(2):1-5. DOI: 10.9734/AJMAH/2017/30905.
11. Thapa S, Sapkota LB, Hamal P. Threat of scrub typhus in post-earthquake Nepal. Journal of Chitwan Medical College 2016;6(18):1-5. https://doi.org/10.3126/jcmc.v6i4.16707.
12. Sapkota LB, Thapa S, Subedi ND. Correlation study of Adenosine deaminase and its isoenzymes in type 2 diabetes mellitus. BMJ Open Diabetes Research & Care 2017;5(1):1-14. doi: 10.1136/bmjdrc-2016-000357.
13. Sapkota LB, Pokharel DR, Shukla PS, Thapa S. Diagnostic value of serum Adenosine deaminase and its isoenzymatic activity in Nepalese patients with type 2 Diabetes Mellitus. Medical Science 2016; 4(4):349-53. 
14. Nepal HP, Neopane P, S Rojeet, Gautam R, Paudel R, Shamshul Ansari S, Sony Shrestha S, Thapa S. Biofilm formation and antimicrobial resistance in Klebsiella pneumoniae isolated from patients visiting a tertiary care center of Nepal. Asian Pacific Journal of Tropical Diseases 2017; 7(6): 347-351. https://doi.org/10.12980/apjtd.7.2017D7-15.
15. Sapkota LB, Thapa S. Correlation between glycemic parameters and lipid profile in type 2 diabetic patients attending tertiary care centre in Central region of Nepal. Journal of Chitwan Medical College 2017;7(19):20-24. https://doi.org/10.3126/jcmc.v7i1.17365.
16. Thapa S, Sapkota LB. Bacteriological assessment of stethoscopes used by healthcare workers in a tertiary care centre of Nepal. BMC Research Notes 2017;10(1):353. doi: 10.1186/s13104-017-2677-7.
17. Thapa S, Gokhale S, Sharma AL, Sapkota LB, Ansari S, Gautam R, Shrestha S and Neopane P. Burden of bacterial upper respiratory tract pathogens in school children of Nepal. BMJ Open Respiratory Research 2017:4(1);1-22. http://dx.doi.org/10.1136/bmjres-2017-000203.
18. Thapa S, Sapkota LB. Changing Trend of Neonatal Septicaemia and Antibiotic Susceptibility Pattern of Isolates in Nepal. International Journal of Pediatrics 2019:3784529;1-7. doi: 10.1155/2019/3784529.
19. Sapkota LB, Thapa S. Pattern of dyslipidaemia in type 2 diabetic patients attending tertiary care centre of Nepal. EC Endocrinology and Metabolic Research 2019:4(9);15-22.
20. Thapa S, Hamal P, Chaudhary NK, Sapkota LB, Singh JP. Burden of scrub typhus among patients with acute febrile illness attending tertiary care hospital in Chitwan, Nepal. BMJ Open 2020;10:e034727. doi: 10.1136/bmjopen-2019-034727

Undergoing research projects as a Principal Investigator:

• Scrub Typhus, ESBL, urinary pathogens among diabetics and non-diabetics.

• Manipal MD Microbiology Scholarship on Merit Basis, 2011
• American Society of Microbiology 2017, Young Scientist Travel Grant Award.
• Travel Grant Awardee; LMCE 2018 (Laboratory Medicine Congress & Exhibition) & KSLM 59th Annual Meeting, Seoul, Korea. 
• Best Paper Award, America Nepal Medical Foundation, First International Microbiology Conference of NACM, 2019 held in Kathmandu, Nepal.

• Actively participated in workshop on “Faculty development programme- Medical Teaching Technology’ held at MCOMS, Pokhara.
• Actively participated in “Workshop on Problem Based Learning (PBL)” from 9th to 11th December 2013 held at MCOMS, Pokhara
• Participation on “Research Methodology Workshop” held from 16th to 27th July 2012 conducted by Department of Community medicine at MTH, Pokhara.
• Participation on “National workshop on laboratory based surveillance of antimicrobial resistance and application of WHONET in laboratory data management” held from 30th Sep to 5th Oct 2012 conducted by Department of Microbiology at MCOMS, Pokhara. 
• Actively participated in workshop on “Scientific writing and publishing - ASM” held at Technical college, Pokhara.
• Participation on “National workshop on laboratory based surveillance of antimicrobial resistance and application of WHONET in laboratory data management” held from 22nd to 27th Sep 2013 conducted by Department of Microbiology at MCOMS, Pokhara.

• Successfully completed Teachers Training Faculty Development Workshop “Embracing Innovations for Enhancing Students Learning” conducted by Chitwan Medical College Teaching Hospital (CMCTH) from 26th to 31st July, 2015.

• Participation in CME on “Post earthquake challenges of infectious diseases” organised by Nepalese Association of Clinical Microbiologists, 27th May 2016, Kist Medical College Teaching Hospital, Lalitpur, Nepal and has earned 7.0 Educational credit points by Nepal Medical Association.

• Participated in workshop on “Competency Based Medical Education (CBME) Curriculum” organized by Chitwan Medical College Teaching Hospital (CMCTH) held on September 10, 2016.

• Completed 6 days Faculty development workshop “Training of Trainers” on Helping Students Learn ETHICS organized by Chitwan Medical College Teaching Hospital (CMCTH) held from September 18-23, 2016.

• Awarded certificate upon successful completion of the “Micro Research Workshop” conducted in collaboration with Chitwan Medical College Teaching Hospital (CMCTH), Nepal and Micro Research International, Canada from November 27 to December 5, 2017 and has earned 14.0 Educational credit points by Nepal Medical Association.

• Oral presentation on “Burden of Scrub Typhus among patients with acute febrile illness attending Chitwan Medical College Teaching Hospital” in the 1st International Microbiology Conference “Man Microbes Machine, finding solution together” Organized by Nepalese Association of Clinical Microbiologists, 12-13 September 2019, Kathmandu, Nepal. 

• Poster presentation on “Changing Trend of Neonatal Septicemia And Antibiotic Susceptibility Pattern of Isolates In Nepal” in the 1st International Microbiology Conference “Man Microbes Machine, finding solution together” Organized by Nepalese Association of Clinical Microbiologists, 12-13 September 2019, Kathmandu, Nepal.

• Presented and Attended – Culture seminars, Topic presentation, Journal clubs, Topic discussion, Continuing Medical Education (CME) and scientific lectures.

Completion of community oriented compulsory rotatory internship programme (COCRIP-BPKIHS).

• Member of “American Society of Microbiology”

• Life long Member of “Nepalese Association of Clinical Microbiologists”